| Culturing P. falciparum in vitro |
| – Basic Culture Maintenance |
| – Making a Smear of Malaria Culture |
| – Making Media for Culture |  |
| – Washing Blood |
| – Culture Synchronization |
| – Thawing Malaria Culture |
| – Freezing Malaria Strains |
| – SYBR Green Growth Assay |
| Transfection |
| – Transfection of P. falciparum |
| Metabolomics Protocols |  |
| – Testing for Mycoplasma (CRITICAL) |
| – Metabolite Extraction for LC/MS (short version) |
| – Metabolite Extraction for LC/MS (detailed version) |
| – Media Extraction for LC/MS |
| Labeling of RNA with 4-thiouracil |
| – RNA Analysis by Biosynthetic Labeling |
| Nucleic Acid Isolation |
| – Malaria Total RNA Isolation Trizol |
| – DNA Miniprep for Cloning or Transfections |
| – Genomic DNA isolation |  |
| – Genomic DNA isolation (DNeasy) |
| Cloning |
| – Ilsa’s Guide to Cloning |
| PCR Procedures | |
| – Colony PCR | |
| – Polymerase Chain Reaction for P. falciparum | |
| Protein Procedures | |
| – Isolation of P. falciparum proteins (Saponin lysis) | |
| – Isolation of P. falciparum nuclear proteins | |
| – Purification of GST-fused proteins | |
| – Tricine Polyacrylamide Protein Gel | |
| – Laemmli Protein Gel | |
| – Silver staining protein gels | |
| – Electrophoretic Mobility Shift Assays (Gel-shifts) | |
| Microarray Procedures | |
| – RT and Amino-allyl Incorporation | |
| – Genomic DNA Labeling | |
| – NEW: cDNA Labeling for Agilent Arrays | |
| – NEW: Hybridization and Washing for Agilent Arrays | |
| – cDNA Labeling Hybridization and Washing | |
| – Malaria Microarray Post Processing | |
| – NEW: Protein Binding Microarray (PBM) Double Stranding | |
| – NEW: Protein Binding Microarray (PBM) Hybing and Scanning | |
| Making Chemically Competent E. Coli Cells | |
